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virusPHIX™ the solution to a universal industry problem

Major deficiencies of current Virus Transport Media (VTM’s) are that they are variously toxic, incompletely inactivate viruses or are poor stabilisers of RNA and proteins, precluding their use for home-testing, transport, storage and safe handling. We have completed development of a solution that uniquely allows the inactivation and preservation of viruses at the point of sampling, whatever the virus and whatever the downstream application. Uniquely, virusPHIX™ is compatible with both nucleic acid (e.g. PCR) and antigen (e.g. Ag lateral flow) based SARS-CoV-2 tests. This buffer also slots faultlessly into your workflow as follows.

virusPHIX™ workflow for integrated SARS-CoV-2 (Covid-19) testing

Specific technical advantages of virusPHIX™

  1. Simultaneously inactivates and preserves clinical samples in one easy step
  2. Completely inactivates >10log7 SARS-CoV-2 in 10 minutes at 20°C
  3. Universal inactivation of 10 tested viruses (Pasteur Institute, CDC, BlueTest)
  4. Kills 3 clinical strains of Mycobacterium tuberculosis in 10 minutes (WEHI)
  5. Direct to LAMP and Lateral Flow assay compatibility (Qnostics UK)
  6. Validated with 8 different SARS-CoV-2 lateral flow assays (Qnostics UK)
  7. Stabilises SARS-CoV-2 Antigen for at least 6 weeks (Qnostics, UK)
  8. Robust SARS-CoV-2 RNA stabilisation for 42 days (Qnostics)
  9. Demonstrated HCV RNA stabilisation for 1 year at 20°C
  10. Universal sample compatibility including swabs and saliva
  11. Proven 100 copy LOD using ThermoFisher KingFisher™ and bioMerieux Nuclisens™
  12. Effortless integration with front-end nucleic acid purification platforms including RNeasy™, QIAsymphony™, QIAcube™, Nuclisens™, GeneXpert™ and Filmarray™
  13. Tested by UK Lighthouse SARS-CoV-2 testing laboratories
  14. Facilitates safe logistics via ambient temperature shipping and storage
  15. Contains no formaldehyde, chaotropes, alcohol or other hazardous chemicals
  16. Green and very low toxicity (LD50 >20g/kg), low volatility and non-flammable
SARS-COV-2 Inactivation Results with virusPHIX™
SARS-COV-2 Inactivation Results with virusPHIX-P9™

1-month 20°C Stabilisation of West Nile Virus and SARS-CoV-2 RNA in Sputum using virusPHIX™ (QIAamp purification and RT-qPCR)

42 Days SARS-CoV-2 RNA Stabilisation at 20°C or 37°C using virusPHIX-P9™

1-Year 20°C Stabilisation of 2 Strains of HCV in Plasma using virusPHIX™

RT-qPCR Limit of Detection (LOD) Study of SARS-CoV-2 in aqueous buffer compared with Inactivating Buffer virusPHIX™ (1-week stabilisation at 20°C)