Cell & tissue fixation
the solution to a universal industry problem
In addition to its proven biomolecule stabilisation properties, vivoPHIX is an exceptionally effective universal cell and tissue fixative that has been successfully used for samples as diverse as plants, parasites, invertebrates and mammals. All tested mammalian tissues types have been fixed with vivoPHIX including fat rich tissues such as adipose and brain, protein rich such as skeletal and cardiac muscle, and standard tissues types including pancreas, liver and kidney. Fixed tissues can be used for standard paraffin embedding, sectioning and staining for H&E, IHC, IF, RNAscope, GFP imaging and FISH.
Multifunctional staining
Diverse detection case study; Various vivoPHIX fixed sample types were imaged using IHC (anti-SMA with mouse kidney), IF (anti-ki67 with mouse stomach), ISH (RNAscope with mouse stomach), IF (Trypanosomes), RFP (Plasmodium), FISH (nascent transcripts in mouse stem cell nuclei), IHC (anti-CD20 with Zika virus infected spleen), IF (anti-FP and DAPI staining with mouse skin), crystal violet staining (human pancreatic adenocarcinoma). Thanks to Maud Borensztein; Roberto Bandiera; Martyna Popis, University of Cambridge; Giuseppe Diaferia, Istituto Europeo di Oncologia; Derrick Robinson, Uni. Bordeaux; Virginia Howick, Sanger Institute; Debbie Ferguson, NIBSC.
(1) Immunohistochemistry (IHC)
Simple mouse (i) brain, (ii) kidney and (iii) intestine fixation with vivoPHIX followed by standard paraffin embedding, sections were stained with anti-Smooth Muscle Actin (SMA).
(2) Immunohistochemistry (IHC)
Simple mouse (i) & (ii) kidney, and (iii) brain fixation with vivoPHIX followed by standard paraffin embedding, sections were used for IHC and stained with anti-b-catenin/
(3) Immunohistochemistry (IHC)
Simple (i) lung, (ii) kidney or (iii) spleen fixation with vivoPHIX followed by standard paraffin embedding, after epitope retrieval with HIER1 for 30 minutes, sections were used for IHC and stained with anti-CD20. Thanks to Dr. D. Ferguson, NIBSC, UK.
Immunofluorescence (IF)
vivoPHIX fixed mouse stomach, paraffin embedded and stained with anti-ki67 (nuclear) antibody and a fluorescent secondary antibody. Thanks to Dr. I. Pshenichnaya, University of Cambridge, UK.
Fluorescent Protein (RFP & GFP)
vivoPHIX fixed fluorescent proteins expressed in (i) Plasmodium (RFP), and (ii) Drosophila larvae (GFP), maintain their fluorescence for several hours in vivoPHIX. Thanks to G. Howick and M. Lawniczak, Sanger Institute, UK, and D. Xu, University of Oxford.
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vivoPHIX™, genoPHIX™, virusPHIX™ and virusPHIX-P9™ are patented next generation cell and tissue fixatives, biomolecule stabilisers with powerful virus and bacterial inactivation properties.
Our reagents are universally compatible with all sample types including viruses, bacteria, fungi, parasites, plants and animal cells and tissues. Both liquid and solid samples are rapidly and completely rendered fixed, stabilised and inactivated.
RNAssist technology supports a wide range of life science applications, including tissue fixation and dissociation, single-cell multiomics (scRNA-seq, CITE-seq), and virus inactivation and transport for enhanced diagnostic testing.
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