vivoPHIX™: our deep eutectic solvent

vivoPHIX™ is a patented deep eutectic solvent (DES) reagent developed by RNAssist to stabilise RNA immediately at the point of collection, before tissue fixation or dissociation.

It uses a non-enzymatic, non-crosslinking fixation method to preserve RNA, DNA, proteins, post-translational modifications, and cellular morphology.

The reagent is compatible with a wide range of sample types including human, animal, plant, bacterial, and viral materials, as well as blood and tissues.

A published protocol using vivoPHIX™ demonstrated high-quality single-cell RNA sequencing from mosquito and sandfly midguts, recovering clean transcriptomes without enzymatic stress or cold-chain constraints.

Key benefits of vivoPHIX™

  • Enables single-cell and multi-omic workflows such as scRNA-seq and CITE-seq by preserving cell integrity and reducing doublets or debris.

  • Retains in vivo transcriptional and signalling states, helping protect key molecular features like phospho-proteins and cellular morphology.

  • Maintains RNA integrity in tissue and cell samples stored for up to 29 days at room temperature or 4 °C.

  • Supports room-temperature transport and long-term storage, removing the need for cold-chain logistics and enabling field-based sampling.

  • Designed to be safe and easy to use, with low toxicity, non-flammability, and no requirement for fume hoods.

Research applications of vivoPHIX™

vivoPHIX™ has been used successfully by research institutes working across insect and mammalian systems, including applications in bone marrow, retina, liver, gut, skin, and midgut dissociation workflows.

Recent studies have shown that it preserves biomolecules and in vivo signalling even under storage or transport conditions that typically compromise sample quality.

RNAssist has partnered with Rapid Labs to make vivoPHIX™ available internationally, supporting labs conducting spatial and single-cell analysis where conventional stabilisation methods often fail.


RNAssist technology supports a wide range of life science applications, including tissue fixation and dissociation, single-cell multiomics (scRNA-seq, CITE-seq), and virus inactivation and transport for enhanced diagnostic testing.

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