Using vivoPHIX to stabilise bacteria from carnivorous plants in the jungle

vivoPHIX has many applications – wherever your research may take you. Here are some photos from a recent project in South East Asia, where we were collecting bacteria from carnivorous plants. It can be difficult to get the required permits for collecting and transporting samples – whether it be bacteria, seeds or plants. To help,… Continue reading Using vivoPHIX to stabilise bacteria from carnivorous plants in the jungle

Partnering with a pan European nematode group, NERVSPAN

We are delighted to be a partner for the EU-funded NERVSPAN research community that will develop an integrative approach to study various aspects of neuronal physiology throughout the lifespan of males and hermaphrodite worms. The simplicity of the 302-node nervous system of the nematode C.elegans, makes this an attractive model organism for neuroscientists, however more work… Continue reading Partnering with a pan European nematode group, NERVSPAN

RNAssist sponsors the 23rd Genomic Standards Consortium Meeting

We are delighted to announce that we are sponsoring The Genomic Standards Consortium (GSC)’s 23rd meeting.  The meeting will be held in Bangkok, Thailand, from August 7th to 11th, 2023, hosted by the Faculty of Medicine Siriraj Hospital at Mahidol University and the National Center for Genetic Engineering and Biotechnology (BIOTEC/NSTDA), the event will have a… Continue reading RNAssist sponsors the 23rd Genomic Standards Consortium Meeting

Two of RNAssist’s molecular stabilsation reagents will be taken to the International Space Station

We are delighted that we can make a small contribution to the important work of Dr. Scott Tighe, Technical Director at the Advanced Genomics Lab at the University of Vermont, NASA-ISS uTitan Payload developer and Group leader Extreme Microbiome Project.    In December 2023, two of RNAssist’s molecular stabilisation reagents will be taken to the International Space Station (ISS) on SpaceX Dragon… Continue reading Two of RNAssist’s molecular stabilsation reagents will be taken to the International Space Station

Fixation Before Dissociation Using a Deep Eutectic Solvent Preserves In Vivo States and Phospho-Signaling in Single-Cell Sequencing

Single-cell RNA sequencing presents an opportunity to deconstruct cellular networks but is limited by the loss of biological information. In this study, a new method of fixation before dissociation using a deep eutectic solvent (DES) is presented.   This method enables multiple domains of in vivo biological data, including morphology, RNA, proteins and posttranslational modifications to… Continue reading Fixation Before Dissociation Using a Deep Eutectic Solvent Preserves In Vivo States and Phospho-Signaling in Single-Cell Sequencing

Profiling the Microbial Communities of Brooks Cave in Pennington County, South Dakota

Studies on cave bacteria have been a valuable research area in both geology and biology, providing insights into the evolution of antibiotic resistance, the role of microbes in cave formation, and the impact of human travel on cave environments. In this study, the authors investigated the microbial communities in Brooks Cave, a perched lake in… Continue reading Profiling the Microbial Communities of Brooks Cave in Pennington County, South Dakota

Taking Insect Immunity to the Single-Cell Level

This research paper used the RNAssist’s vivoPHIX™ system to examine the intricacies of insect innate immunity. Sarah Merkling et al., aimed to gain a deeper understanding of the immune responses in insects at the level of specific organs and cell populations, with the goal of improving our overall knowledge of tissue- or cell-specific immune processes… Continue reading Taking Insect Immunity to the Single-Cell Level

Expression of oxysterols in human lenses: Implications of the sterol pathway in age-related cataracts

Lanosterol is implicated in cataract formation – it has been proposed to help maintain lens transparency by inhibiting the formation of protein aggregates.  Their data confirms that lanosterol synthase and the sterol pathway are upregulated in cataractous lenses.  In the study, Dr Avila’s laboratory used lenses that were treated with the stabliser and tissue fixative… Continue reading Expression of oxysterols in human lenses: Implications of the sterol pathway in age-related cataracts

Determination and localisation of Lanosterol Synthase in human cataractous lenses and their relationship with αA crystallin proteins

Dr Avila’s laboratory has used vivoPHIX™ to prepare human cataract crystallin proteins.  The study aimed to determine the expression of Lanosterol Synthase in human cataractous lenses and their association with α A crystallin protein.  Read the research

Tools for connectomics in C.elegans

Nicholas Barry at the MIT Media Lab used vivoPHIX™ for preparing nematodes for fluorescence studies in work titled “Tools for connectomics in C.elegans“ The simplicity of the 302-node nervous system of the nematode C.elegans, makes this an attractive model organism for neuroscientists, however more work is required to fully understand and characterise the function of… Continue reading Tools for connectomics in C.elegans